转CryIA基因抗虫棉花棉籽的诱变活性研究
页数：10 字数：7233
转CryIA基因抗虫棉花棉籽的诱变活性研究摘 要：分别以环磷酰胺和氟化钠作为阳性诱变剂，以普通棉的棉籽粉及棉籽油作为对照物，同时设阴性对照组，以成年封闭群昆明种雄性小鼠和斑马鱼(Branchynanio rerio)为实验材料，将棉籽粉或棉籽油掺入饲料中饲喂小鼠或斑马鱼，检测小鼠骨髓嗜多染红细胞（Polychromatic erythrocytes, PCE）微核率（Micronucleus frequency, MN‰）和精子畸变率（Sperm malformation rate），以及斑马鱼外周血红细胞（Peripheral erythrocytes）微核率。结果表明：在本实验条件下，转CryIA基因抗虫棉与普通棉的棉籽粉及棉籽粉油对小鼠体细胞和性细胞均未显示出诱变活性，且没有剂量效应。腹腔注射氟化钠可以诱导斑马鱼外周血红细胞微核率的显著升高；转CryIA基因抗虫棉棉籽粉粉和普通棉棉籽粉粉一样，对斑马鱼外周血红细胞微核率无明显影响。鱼类饲料中添加常规量的转CryIA基因抗虫棉棉籽粉不会诱发微核产生。
关键词：Bt抗虫棉,小鼠,斑马鱼,微核率,精子畸变率
Study on Mutagenicity of Cotton Seed Powder and Cotton Seed Oil
of Transgenic CryIA Cotton*
Zhang Ning，Chen Mingnan，Ma Jixia，Li Songgang，Xu Chongren**
（Department of Environmental Biology and Ecology, College of Life Sciences，
Peking University，100871）
Abstract：The mutagenicity of seed powder and seed oil of transgenic CryIA cotton to mammals and fish was evaluated in this study. The raw cotton seed powder or seed oil was mixed into mice and fish’s diet. Cyclophosphamide and NaF were used as positive controls respectively. Regular cotton seed powder and seed oil were used as negative controls. The micronucleus frequency in mice bone marrow polychromatic erythrocytes and in eripheral erythrocytes of Zebrafish (Branchynanio rerio) were assayed in this study. The sperm malformation rate in mice was also investigated. The results showed that, the seed powder and seed oil of transgenic CryIA cotton lines and regular cotton showed no cytomutagenicity or genomutagenicity on mice. No dose effect was observed. NaF can induce significant increase of micronucleus frequency in eripheral erythrocytes of Zebrafish. Neither t
