GSH对低温胁迫下枇杷幼果叶绿体AsA-GSH循环代谢的影响
目 录
1 引言 2
2 材料与方法 2
2.1 供试材料 2
2.2 主要试剂与仪器 2
2.3 测定项目与方法 2
2.4 数据分析 3
3 结果与分析 3
3.1 GSH对低温胁迫下枇杷幼果叶绿体GSH含量的影响 3
3.2 GSH对低温胁迫下枇杷幼果叶绿体AsA含量的影响 3
3.3 GSH对低温胁迫下枇杷幼果叶绿体中MDHAR活性的影响 3
4 讨论 3
5 结论 4
致谢 4
参考文献 4
附录 5
摘要：探讨GSH在提高枇杷(Eriobotrya japonica Lindl.)幼果抗寒性方面的应用。以3年生“早钟6号”枇杷(Eriobotrya japonica Lindl.cv. Zaozhong NO.6)容器苗为试材，研究不同质量浓度谷胱甘肽(GSH)处理对﹣3℃条件下枇杷幼果叶绿体AsA-GSH循环代谢的影响及其在抗低温胁迫中的作用。低温胁迫下枇杷幼果叶绿体抗氧化剂还原型谷胱甘肽(GSH)和抗坏血酸(AsA)含量下降, 多种重要酶的活性受到不同程度的抑制。采用GSH处理可显著提高低温胁迫下枇杷幼果叶绿体GSH等的活性，促进AsA-GSH循环，在枇杷幼果抵抗低温胁迫中发挥作用。
关键词：叶绿体; 还原型谷胱甘肽(GSH); 低温胁迫; AsA-GSH循环代谢
Effects of GSH on AsA-GSH Circulation Metabolism in Chloroplasts of Young Loquat Fruits Under Low Temperature Stress
Abstract: The paper studied the application of reduced glutathione (GSH) in increasing the chilling resistance abilities of young loquat (Eriobotrya japonica Lindl.cv. Zaozhong NO.6) fruits. The container seedling of three-year-old loquat were treated with 100, 300 and 500 mg·L-1 of GSH. The study was to investigate the effects of exogenous GSH on AsA-GSH circulation metabolism in chloroplasts of young loquat fruits under low temperature stress, and its effects on the resistance to low temperature stress. The results showed that the contents of GSH and ascorbic acid(AsA) in chloroplasts decreased while the activities of some important enzymes also decreased when the young loquat fruits were under low temperature stress. The treatment with 100 mg·L-1 of GSH on young loquat fruits could significantly improve the GSH, AsA contents and APX, GR, MDHAR activities. Furthermore, the treatment promoted AsA-GSH circulation metabolism, and improved the ability of free radicals to scavenge in chloroplasts. The results demonstrated that exogenous GSH played a role in the low temperature stress resistance of young loquat fruits.
Keywords: chloroplast; reduced glutathione (GSH); low temperature strees; AsA-GSH circulation metabolism
