毕业论文甲醛胁迫对洋葱内表皮细胞凋亡的诱导.doc

目录
1.论文题目、姓名、学院........................................... 2
2. 中文摘要、英文摘要...........................................2-3
3. 引言..........................................................3
4.实验材料与方法步骤:.............................................3
4.1实验材料..................................................................................... 3
4.2方法步骤：.................................................3
4.2.1材料处理...............................................3
4.2.1.1预处理...........................................3
4.2.1.2取材.............................................4
4.2.1.3处理.............................................4
4.2碱性品红溶液染色及形态学观测................................4
4.3细胞凋亡计数统计............................................4
5.结果与分析......................................................4
5.1细胞凋亡的形态学变化.....................................4-5
5.2细胞凋亡率................................................5
6.讨论..........................................................5-6
7.参考文献......................................................6-7
8.致谢...........................................................7





甲醛胁迫对洋葱内表皮细胞调往的诱导
付涛，生命科学学院

摘要： 细胞凋亡是当今环境毒理学领域的研究热点之一．为了探讨甲醛能否诱导细胞凋亡，以洋葱内表皮细胞为实验材料，通过不同浓度的甲醛处理一段时间后，应用形态学观察、显微镜观察实验检测了洋葱内表皮细胞凋亡情况．实验结果表明低浓度甲醛 (≤0．2mmol•L)处理洋葱内表皮细胞一段时间后，对细胞生长有显著的抑制作用 ，但对洋葱内表皮细胞形态改变不大，且对细胞核DNA没有损伤；而高浓度甲醛 (≥0．4mmol•L)处理细胞 一段时间后，对细胞生长有极显著的抑制作用，细胞形态逐渐变的模糊不清，并有少量细胞萎缩成圆形， 且会造成细胞核DNA有规律的断裂，细胞凋亡率随甲醛浓度的增加而增大．以上实验结果显示，高浓度甲醛处理洋葱内表皮细胞一段时间后，会诱导细胞凋亡；并且随甲醛浓度的升高凋亡率逐渐 增大，呈一定的剂量一效应关系．
关键词：细胞凋亡；甲醛；洋葱内表皮细胞；显微镜

Abstract： Nowadays apoptosis has gained much interest in the environmentaltoxicology．In order to explore whether formaldehyde could induce apoptosis or not， Onion epidermal cells were treated with different concentrations of fomraldehyde for a period of time ．Morphologic observation、Microscopic observation of experimental were applied to detect the apoptosis of Onion epidermal cells in this experiment．Results showed that Onion epidermal cells’ prolifeation was significantly inhibited alter treated with FA atlow concentrations (≤ 0．2mmol/L )ina period of time ，while morphological change of cells was negligible，and no significant damage of cellular DNA wasobserved under the conditions．However，Onion epidermal cells’ proliferation was statistically significantly inhibited after treated with FA a thigh concentrations(≥0．4mmol/L)ina period of time ，nada small amount of cells shrank intorotundity．Meanwhile，cell nucleus DNA was broken intoregularfragment，and apoptosis rate was increased with the elevation of FA concentrations．These experiments showed hat the treatment of Onion epidermalcells by FA a thigh concentrations of r a period of time might induce cell apoptosis；andtherewasna obviously dose—depend entre lationship between FA concentrations and apoptosisrate．
Key words：apoptosis； formaldehyde；Onion epidermal cells；microscope